Difference between revisions of "Part:BBa K1796011"
Line 18: Line 18:
  
 
==Parameter of Protein==
 
==Parameter of Protein==
−
<p> Number of amino acids: 475 </p>
+
<p> Number of amino acids: 1434 </p>
 
<p> </p>
 
<p> </p>
−
<p> Molecular weight: 51557.1 </p>
+
<p> Molecular weight: 118547.3 </p>
 
<p> </p>
 
<p> </p>
−
<p> Theoretical pI: 8.13 </p>
+
<p> Theoretical pI: 4.97 </p>
 
<p> </p>
 
<p> </p>
−
<p> Amino acid composition:  </p>
+
<p> Amino acid composition:聽 </p>
 
<p> </p>
 
<p> </p>
−
<p> Ala (A) 46      9.7% </p>
+
<p> Ala (A) 316      22.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Arg (R) 31       6.5% </p>
+
<p> Arg (R)   0       0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Asn (N) 10       2.1% </p>
+
<p> Asn (N)   0       0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Asp (D) 22       4.6% </p>
+
<p> Asp (D)   0       0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Cys (C)   8      1.7% </p>
+
<p> Cys (C) 365      25.5% </p>
 
<p> </p>
 
<p> </p>
−
<p> Gln (Q) 14       2.9% </p>
+
<p> Gln (Q)   0       0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Glu (E) 25       5.3% </p>
+
<p> Glu (E)   0       0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Gly (G) 42      8.8% </p>
+
<p> Gly (G) 402      28.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> His (H) 15       3.2% </p>
+
<p> His (H)   0       0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Ile (I) 21       4.4% </p>
+
<p> Ile (I)   0       0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Leu (L) 56      11.8% </p>
+
<p> Leu (L)   0      0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Lys (K) 18       3.8% </p>
+
<p> Lys (K)   0       0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Met (M) 13       2.7% </p>
+
<p> Met (M)   0       0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Phe (F) 15       3.2% </p>
+
<p> Phe (F)   0       0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Pro (P) 24       5.1% </p>
+
<p> Pro (P)   0       0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Ser (S) 44       9.3% </p>
+
<p> Ser (S)   0       0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Thr (T) 24       5.1% </p>
+
<p> Thr (T) 351      24.5% </p>
 
<p> </p>
 
<p> </p>
−
<p> Trp (W)  8       1.7% </p>
+
<p> Trp (W)  0       0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Tyr (Y)  9       1.9% </p>
+
<p> Tyr (Y)  0       0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Val (V) 30       6.3% </p>
+
<p> Val (V)   0       0.0% </p>
 
<p> </p>
 
<p> </p>
 
<p> Pyl (O)  0      0.0% </p>
 
<p> Pyl (O)  0      0.0% </p>
Line 76: Line 76:
 
<p> (X)  0          0.0% </p>
 
<p> (X)  0          0.0% </p>
 
<p> </p>
 
<p> </p>
−
<p> Total number of negatively charged residues (Asp + Glu): 47 </p>
+
<p> Total number of negatively charged residues (Asp + Glu): 0 </p>
−
<p> Total number of positively charged residues (Arg + Lys): 49 </p>
+
<p> Total number of positively charged residues (Arg + Lys): 0 </p>
 
<p> </p>
 
<p> </p>
−
<p> Atomic composition:Carbon      C              2282 </p>
+
<p> Atomic composition:Carbon      C              4251 </p>
−
<p> Hydrogen    H        3634 </p>
+
<p> Hydrogen    H        7070 </p>
−
<p> Nitrogen    N         648 </p>
+
<p> Nitrogen    N         1434 </p>
−
<p> Oxygen      O         671 </p>
+
<p> Oxygen      O         1786 </p>
−
<p> Sulfur      S           21 </p>
+
<p> Sulfur      S         365 </p>
 
<p> </p>
 
<p> </p>
−
<p> Formula: C2282H3634N648O671S21Total number of atoms: 7256 </p>
+
<p> Formula: C4251H7070N1434O1786S365Total number of atoms: 14906 </p>
 
<p> </p>
 
<p> </p>
−
<p> Extinction coefficients:Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water. </p>
+
<p> Extinction coefficients:This protein does not contain any Trp residues. Experience shows that </p>
 +
<p> this could result in more than 10% error in the computed extinction coefficient. </p>
 
<p> </p>
 
<p> </p>
−
<p> Ext. coefficient    57910 </p>
+
<p> Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water. </p>
−
<p> Abs 0.1% (=1 g/l)  1.123, assuming all pairs of Cys residues form cystines </p>
+
 
<p> </p>
 
<p> </p>
 +
<p> Ext. coefficient    22750 </p>
 +
<p> Abs 0.1% (=1 g/l)  0.192, assuming all pairs of Cys residues form cystines </p>
 
<p> </p>
 
<p> </p>
−
<p> Ext. coefficient   57410 </p>
+
<p> </p>
−
<p> Abs 0.1% (=1 g/l)  1.114, assuming all Cys residues are reduced </p>
+
<p> Ext. coefficient       0 </p>
 +
<p> Abs 0.1% (=1 g/l)  0.000, assuming all Cys residues are reduced </p>
 
<p> </p>
 
<p> </p>
 
<p> Estimated half-life:The N-terminal of the sequence considered is G (Gly). </p>
 
<p> Estimated half-life:The N-terminal of the sequence considered is G (Gly). </p>
Line 103: Line 106:
 
<p> </p>
 
<p> </p>
 
<p> </p>
 
<p> </p>
−
<p> Instability index:The instability index (II) is computed to be 40.14 </p>
+
<p> Instability index:The instability index (II) is computed to be 44.79 </p>
 
<p> This classifies the protein as unstable. </p>
 
<p> This classifies the protein as unstable. </p>
 
<p> </p>
 
<p> </p>
 
<p> </p>
 
<p> </p>
 
<p> </p>
 
<p> </p>
−
<p> Aliphatic index: 91.22 </p>
+
<p> Aliphatic index: 22.04 </p>
 
<p> </p>
 
<p> </p>
−
<p> Grand average of hydropathicity (GRAVY): -0.062 </p>
+
<p> Grand average of hydropathicity (GRAVY): 0.750 </p>

Revision as of 01:44, 19 September 2015

nifN promoted from Paenibacillus sp. WLY78

Function: scaffold gene of nitrogen fixation system

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 319
    Illegal PstI site found at 890
    Illegal PstI site found at 961
    Illegal NgoMIV site found at 648
    Illegal AgeI site found at 25
    Illegal AgeI site found at 147
    Illegal AgeI site found at 766
    Illegal AgeI site found at 987
  • 1000
    COMPATIBLE WITH RFC[1000]


Parameter of Protein

Number of amino acids: 1434

Molecular weight: 118547.3

Theoretical pI: 4.97

Amino acid composition:聽

Ala (A) 316 22.0%

Arg (R) 0 0.0%

Asn (N) 0 0.0%

Asp (D) 0 0.0%

Cys (C) 365 25.5%

Gln (Q) 0 0.0%

Glu (E) 0 0.0%

Gly (G) 402 28.0%

His (H) 0 0.0%

Ile (I) 0 0.0%

Leu (L) 0 0.0%

Lys (K) 0 0.0%

Met (M) 0 0.0%

Phe (F) 0 0.0%

Pro (P) 0 0.0%

Ser (S) 0 0.0%

Thr (T) 351 24.5%

Trp (W) 0 0.0%

Tyr (Y) 0 0.0%

Val (V) 0 0.0%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 0

Total number of positively charged residues (Arg + Lys): 0

Atomic composition:Carbon C 4251

Hydrogen H 7070

Nitrogen N 1434

Oxygen O 1786

Sulfur S 365

Formula: C4251H7070N1434O1786S365Total number of atoms: 14906

Extinction coefficients:This protein does not contain any Trp residues. Experience shows that

this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 22750

Abs 0.1% (=1 g/l) 0.192, assuming all pairs of Cys residues form cystines

Ext. coefficient 0

Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is G (Gly).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 44.79

This classifies the protein as unstable.

Aliphatic index: 22.04

Grand average of hydropathicity (GRAVY): 0.750