Difference between revisions of "Part:BBa K1688002"

Revision as of 21:44, 18 September 2015

RhlA Gene

The enzymes responsible for producing mono-rhamnolipids (biosurfactants) are called RhlA and RhlB which are encoded by the genes rhlA and rhlB respectively. The protein encoded by these two make up the subunits of rhamnosyltransferase I. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The rhlA gene coding for the enzyme RhlA creates the fatty acid tail and has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from Pseudomona aeruginosa. The biobrick (Ba_K1688002) is an assembly of RBS with (BBa_K1331001).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 87
Illegal BamHI site found at 647
Illegal XhoI site found at 823
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 312
Illegal BsaI.rc site found at 496

Revision as of 21:16, 18 September 2015 (view source) Oskarwestlin (Talk \| contribs) ← Older edit		Revision as of 21:44, 18 September 2015 (view source) Sangeethabupalan (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K1688002 short</partinfo>		<partinfo>BBa_K1688002 short</partinfo>

−	The enzymes responsible for producing mono-rhamnolipids (biosurfactants) are called RhlA and RhlB which are encoded by the genes <i>rhlA</i> and <i>rhlB</i> respectively. The protein encoded by these two make up the subunits of rhamnosyltransferase I. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The ~~RhlA~~ gene ~~codes~~ for the fatty acid tail and has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from <i>''Pseudomona aeruginosa''</i>. The biobrick ([https://parts.igem.org/Part:BBa_K1688002 Ba_K1688002]) is an assembly of RBS with ([https://parts.igem.org/Part:BBa_K1331001 BBa_K1331001]).	+	The enzymes responsible for producing mono-rhamnolipids (biosurfactants) are called RhlA and RhlB which are encoded by the genes <i>rhlA</i> and <i>rhlB</i> respectively. The protein encoded by these two make up the subunits of rhamnosyltransferase I. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The <i>rhlA</i> gene coding for the enzyme RhlA creates the fatty acid tail and has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from <i>''Pseudomona aeruginosa''</i>. The biobrick ([https://parts.igem.org/Part:BBa_K1688002 Ba_K1688002]) is an assembly of RBS with ([https://parts.igem.org/Part:BBa_K1331001 BBa_K1331001]).