<p> Total number of negatively charged residues (Asp + Glu): 0 </p>
−
<p> Total number of positively charged residues (Arg + Lys): 0 </p>
−
<p> </p>
−
<p> Atomic composition:Carbon C 4334 </p>
−
<p> Hydrogen H 7197 </p>
−
<p> Nitrogen N 1473 </p>
−
<p> Oxygen O 1791 </p>
−
<p> Sulfur S 352 </p>
−
<p> </p>
−
<p> Formula: C4334H7197N1473O1791S352Total number of atoms: 15147 </p>
−
<p> </p>
−
<p> Extinction coefficients:This protein does not contain any Trp residues. Experience shows that </p>
−
<p> this could result in more than 10% error in the computed extinction coefficient. </p>
−
<p> </p>
−
<p> Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. </p>
−
<p> </p>
−
<p> Ext. coefficient 22000 </p>
−
<p> Abs 0.1% (=1 g/l) 0.184, assuming all pairs of Cys residues form cystines </p>
−
<p> </p>
−
<p> </p>
−
<p> Ext. coefficient 0 </p>
−
<p> Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced </p>
−
<p> </p>
−
<p> Estimated half-life:The N-terminal of the sequence considered is C (Cys). </p>
−
<p> </p>
−
<p> The estimated half-life is: 1.2 hours (mammalian reticulocytes, in vitro). </p>
−
<p> >20 hours (yeast, in vivo). </p>
−
<p> >10 hours (Escherichia coli, in vivo). </p>
−
<p> </p>
−
<p> </p>
−
<p> Instability index:The instability index (II) is computed to be 40.53 </p>
−
<p> This classifies the protein as unstable. </p>
−
<p> </p>
−
<p> </p>
−
<p> </p>
−
<p> Aliphatic index: 27.29 </p>
−
<p> </p>
−
<p> Grand average of hydropathicity (GRAVY): 0.829 </p>
Revision as of 22:30, 18 September 2015
nifD promoted from Paenibacillus sp. WLY78
Function: αsubunits of dinitrogease which also called FeMo protein. NifD encode a metallocluster: FeMo-co, a [Mo-7Fe-9S-C-homocitrate] cluster which serves as the active site of substrate binding and reduction.
We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But we were not informed of the error in promotion.After several times failed expriments, we found the problem.We tackled them over the matter, mistakes were corrected, but the correct genes can't arrive on time,it can only arrive after the deadline.
Sequence submit is promoted by the synthesis company, containing a PstI in the gene.
Sequence and Features
Assembly Compatibility:
10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 150
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 150
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1116
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 150
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 150 Illegal AgeI site found at 1141
