Difference between revisions of "Part:BBa K1763000"

Revision as of 21:36, 18 September 2015

Apis mellifera (honeybee) silk fibroin 3

This is the coding region for the honeybee silk protein #3. Previous literature has shown that it is possible to generate honeybee fibers by expressing this protein recombinantly in e. coli and spinning the protein into fibers in vitro It does not contain any of the regulatory elements necessary for protein expression.

Usage and Biology

Because this is just the coding region for the honeybee silk sequence, it is not meant to be expressed on its own. For an expressible honey bee silk biobrick part, please see BBa_K1763007 .

Characterization

1. Protein Expression

• Here is the SDS gel of our expression of the honeybee silk protein. It was purified using [http://wolfson.huji.ac.il/purification/PDF/Protein_Expression_Extraction/NOVAGEN_BugBuster_protein_extraction.pdf this inclusion body protocol]

Fig. 1 Expected size of product is 40.0 kDA

• There are contaminating bands present on the SDS PAGE gel, indicating that the purification is not completely effective.
• We also ran BCA protein concentration assays on our purified honeybee proteins. Based on this assay we were able to get around 12 mg of protein from a 300 ml cell culture.
  • However, this 12 mg might not be composed entirely of honeybee protein because based on the SDS PAGE results, the purification was not 100% complete.

Sequence and Features