Difference between revisions of "Part:BBa K1729002"
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The part sequence corresponds to nucleotides 694840-694739 on chromosome XIV of the ''S. cerevisiae'' S288C reference genome.
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The part sequence corresponds to nucleotides 694840-694739 on chromosome XIV of the ''S. cerevisiae'' S288C reference genome. See also [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1729002 BBa_K1729001]
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[[File:MLS Cap.jpg|900 px|]]
 
[[File:MLS Cap.jpg|900 px|]]

Revision as of 02:54, 19 September 2015

Yeast MRPS12 mitochondrial localization signal

This translational start sequence includes the Kozak and codon-optimized sequence for the mitochondrial localization signal of Saccharomyces cerevisiae mitochondrial ribosomal protein [http://www.yeastgenome.org/locus/S000005319/overview MRPS12]. The cleavage site was predicted based on identification of an R-10 motif (RxFxxTxxxx), which is typical of precursors cleaved sequentially by the yeast mitochondrial processing peptidase and mitochondrial intermediate peptidase (Branda et al.).


The part sequence corresponds to nucleotides 694840-694739 on chromosome XIV of the S. cerevisiae S288C reference genome. See also BBa_K1729001


MLS Cap.jpg

Screen capture from SnapGene®

References

Branda, Steven S et al. "Prediction and Identification of New Natural Substrates of the Yeast Mitochondrial Intermediate Peptidase." J Biol Chem 270 (1995): 27366-73.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]