Difference between revisions of "Part:pSB1C3"

Revision as of 16:31, 27 September 2017

High copy BioBrick assembly plasmid

pSB1C3 is a high copy number plasmid (RFC [10]) carrying chloramphenicol resistance.

The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

pSB1C3 has terminators bracketing its MCS which are designed to prevent transcription from *inside* the MCS from reading out into the vector. The efficiency of these terminators is known to be < 100%. Ideally we would construct a future set of terminators for bracketing a MCS that were 100% efficient in terminating both into and out of the MCS region.

pSB1C3 is the designated Registry shipping plasmid backbone. All submissions for iGEM competitions must be submitted using pSB1C3.

Go to this page for more information on how to ship submissions to iGEM HQ.

Sample Location

The distribution kits contain many samples of pSB1C3 as it is the Registry's main backbone for shipping and maintaining samples. Our recommendation when cloning a part into pSB1C3 is that you can use either linearized plasmid backbone provided in the kits, or pSB1C3 with a reporter (BBa_J04450).

2017 Kit Plate 4 - Well 4D has a sample of BBa_J04450 in pSB1C3.

Usage and Biology

In Spring 2011, pSB1C3 was sequenced using several primers: Pre-R, Suff-F, VF2, VR, and internal primers for the origin and resistance. The reference sequence below matches the most recent sequencing verification of pSB1C3 (w/ BBa_J04450, located in SP 4000 Well 2A).

Sequence and Features