Difference between revisions of "Part:BBa K1820003"
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<p> This promoter region contains a leftward and rightward promoter. Expression is induced from the rightward promoter upon initial phage infection, resulting in transcription of the downstream genes, and translation beginning at the RBS. </p> | <p> This promoter region contains a leftward and rightward promoter. Expression is induced from the rightward promoter upon initial phage infection, resulting in transcription of the downstream genes, and translation beginning at the RBS. </p> | ||
| − | + | <p> This construct has yet to be cloned and expressed in ''L. lactis'', and validated for functionality.</p> | |
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Latest revision as of 16:14, 26 September 2015
P335-Φ31phage genetic switch-divergent promoter + native RBS
A divergent phage-inducible promoter from the genetic switch region of the P335 species lactococcal phage Φ31 (BBa_K1820002), with the native ribosomal binding site (RBS)
Usage and Biology
This promoter region contains a leftward and rightward promoter. Expression is induced from the rightward promoter upon initial phage infection, resulting in transcription of the downstream genes, and translation beginning at the RBS.
This construct has yet to be cloned and expressed in L. lactis, and validated for functionality.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Durmaz E, Madsen SM, Israelsen H, Klaenhammer TR (2002) Lactococcus lactis lytic bacteriophages of the P335 group are inhibited by overexpression of a truncated CI repressor. J Bacteriol 184:6532–6544.