Difference between revisions of "Part:BBa K1819001"
Line 11: Line 11:
 
<p>Figure 1 - Schematic representation of BBa_K1819001 insert</p>
 
<p>Figure 1 - Schematic representation of BBa_K1819001 insert</p>
  
−
<img src="https://static.igem.org/mediawiki/2015/b/b8/Team-Brasil-USP-LCP-PSB1C3.png" width= "300"> </img src>
+
<img src="https://static.igem.org/mediawiki/2015/b/b8/Team-Brasil-USP-LCP-PSB1C3.png" width= "350"> </img src>
 
<p>figure 2 - Gel analysis confirmation of Lcp in pSB1C3</p>
 
<p>figure 2 - Gel analysis confirmation of Lcp in pSB1C3</p>
  

Revision as of 18:51, 18 September 2015

Latex Clearing Protein (Lcp) - rubber degradation

Latex clearing protein (Lcp) from actinomycete Streptomyces sp. strain K30 (GenBank accession code: AAR25849.1) is a 42kDa enzyme which catalyzes rubber cleavage at the cis double bonds to multiple products ranging from C20 tetra-isoprenoid to at least C35 hepta-isoprenoid with measured specific activity of 1.3 mol/min per mg.

It contains an oxidized heme-Fe3+ not bound to dioxygen.


Figure 1 - Schematic representation of BBa_K1819001 insert

figure 2 - Gel analysis confirmation of Lcp in pSB1C3


Please note this part was submitted in pSB1C3, the Registry's standard shipping backbone, according to submission requirements. However, pSB1C3 contains a SacI site and if you want to use this part we recommend to move the part into another plasmid backbone what can be easily done.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 889
    Illegal XhoI site found at 1043
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 535
    Illegal NgoMIV site found at 857
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 438