Difference between revisions of "Part:BBa K1763000"

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<partinfo>BBa_K1763000 short</partinfo>
 
<partinfo>BBa_K1763000 short</partinfo>
  
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This is the coding region for the honeybee silk protein #3 (will update more later)
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This is the coding region for the honeybee silk protein #3. Previous literature has shown that it is possible to generate honeybee fibers by expressing this protein recombinantly in ''e. coli ''and spinning the protein into fibers ''in vitro'' It does not contain any of the regulatory elements necessary for protein expression.
  
  

Revision as of 18:53, 18 September 2015

Apis mellifera (honeybee) silk fibroin 3

This is the coding region for the honeybee silk protein #3. Previous literature has shown that it is possible to generate honeybee fibers by expressing this protein recombinantly in e. coli and spinning the protein into fibers in vitro It does not contain any of the regulatory elements necessary for protein expression.


Usage and Biology

Hello

Characterization

  1. Protein Expression
  • Here is the SDS gel of our first attempt at expression of the honeybee silk protein. The purified protein should be in the lane labeled "inclusion body." It was purified using [http://wolfson.huji.ac.il/purification/PDF/Protein_Expression_Extraction/NOVAGEN_BugBuster_protein_extraction.pdf this inclusion body protocol]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]