Difference between revisions of "Part:BBa K1819001"

Revision as of 18:48, 18 September 2015

Latex Clearing Protein (Lcp) - rubber degradation

Latex clearing protein (Lcp) from actinomycete Streptomyces sp. strain K30 (GenBank accession code: AAR25849.1) is a 42kDa enzyme which catalyzes rubber cleavage at the cis double bonds to multiple products ranging from C20 tetra-isoprenoid to at least C35 hepta-isoprenoid with measured specific activity of 1.3 mol/min per mg.

It contains an oxidized heme-Fe³⁺ not bound to dioxygen.

Figure 1 - Schematic representation of BBa_K1819001 insert

figure 2 - Gel analysis confirmation of Lcp in pSB1C3

Please note this part was submitted in pSB1C3, the Registry's standard shipping backbone, according to submission requirements. However, pSB1C3 contains a SacI site and if you want to use this part we recommend to move the part into another plasmid backbone what can be easily done.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 889
Illegal XhoI site found at 1043
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 535
Illegal NgoMIV site found at 857
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 438

@@ Line 9: / Line 9: @@
 <br>
 <center><img src="https://static.igem.org/mediawiki/2015/f/f4/Brasil-USPLcpscheme.png" width= "150"> </img src>
-<p>Schematic representation of BBa_K1819001 insert</p>
+<p>Figure 1 - Schematic representation of BBa_K1819001 insert</p>
 <img src="https://static.igem.org/mediawiki/2015/b/b8/Team-Brasil-USP-LCP-PSB1C3.png" width= "150"> </img src>
-<p>Gel analysis confirmation of Lcp in pSB1C3</p>
+<p>figure 2 - Gel analysis confirmation of Lcp in pSB1C3</p>
 </center>