Difference between revisions of "Part:BBa K1723008:Experience"
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[B_alt] apo-dCas9-ω (=no sgRNA) <br/> | [B_alt] apo-dCas9-ω (=no sgRNA) <br/> | ||
| − | [Af_alt] | + | [Af_alt] apo-dCas9-ω (=no sgRNA), no reporter<br/> |
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Latest revision as of 20:34, 18 September 2015
Applications of BBa_K1723008
This picture represent the system we want to build using this promoter: a transistor like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.
Effects of dCas9-ω BBa_K1723000 on BBa_K1723005 promoter
promoter strength was assessed using GFPmut2 reporter.
This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117Alt promoter (BBa_K1723005) when provided following sgRNAs:
[A_alt] sgRNA X4 (BBa_K1723007), activating
[R2_alt] sgRNA X0 (BBa_K1723006), inhibiting
[R1_alt] sgRNA X35 (BBa_K1723008), inhibiting
In comparison with:
[B_alt] apo-dCas9-ω (=no sgRNA)
[Af_alt] apo-dCas9-ω (=no sgRNA), no reporter
This chart show the response of the promoter PAM rich URS J23117Alt (BBa_K1723001) in the presence of different complexes sgRNA-dCas9-ω provided by our part collection. Specifically, we see that in presence of the complex BBa_K1723008-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is not activated. Visit our website for more informations: http://2015.igem.org/Team:EPF_Lausanne
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