Difference between revisions of "Part:BBa K808025:Experience"

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<I>Tianjin</I>
 
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===Applications of BBa_K808025===
 
===Applications of BBa_K808025===
 
This part was used by Tianjin 2015 in its Stimulated Plastic Enzymolysis.We aim to enhance the effect of FsC by the addition of hydrophobin and make them into a fusion protein. We could provide abundant data about its expression in E.coli. You could also find its fusion protein in BBa_K1582020, BBa_K1582021,BBa_K1582022 and BBa_K1582023.
 
This part was used by Tianjin 2015 in its Stimulated Plastic Enzymolysis.We aim to enhance the effect of FsC by the addition of hydrophobin and make them into a fusion protein. We could provide abundant data about its expression in E.coli. You could also find its fusion protein in BBa_K1582020, BBa_K1582021,BBa_K1582022 and BBa_K1582023.
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Nanodrop measurements of the purified protein FsC: 0.414mg/mL <br>
 
Nanodrop measurements of the purified protein FsC: 0.414mg/mL <br>
 
·Concentrate FsC with concentration tube and change the buffer from 200mM MCAC to 1X PBS because 200mM MCAC is noxious for enzyme. Final concentration is 2.870mg/mL <br>
 
·Concentrate FsC with concentration tube and change the buffer from 200mM MCAC to 1X PBS because 200mM MCAC is noxious for enzyme. Final concentration is 2.870mg/mL <br>
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Revision as of 19:16, 18 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K808025

This part was improved in 2013 by the iGEM team of the TU Darmstadt. Please take a look at BBa_K1055007 to see the improvement. Among others an online expression detection method was implemented to gain better control over the enzyme production.

User Reviews

UNIQd976d4c067c4e11a-partinfo-00000000-QINU UNIQd976d4c067c4e11a-partinfo-00000001-QINU