Difference between revisions of "Part:BBa K1723006:Experience"
E.Cuillery (Talk | contribs) |
E.Cuillery (Talk | contribs) |
||
| Line 6: | Line 6: | ||
<br/><br/><br/> | <br/><br/><br/> | ||
https://static.igem.org/mediawiki/2015/4/45/EPF_Lausanne_transistor.png | https://static.igem.org/mediawiki/2015/4/45/EPF_Lausanne_transistor.png | ||
| + | |||
| + | <p>promoter strength was assessed using GFPmut2 reporter.</p> | ||
| + | <br/> | ||
<h3>This picture represent the system we want to build using this promoter: a transistor like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.</h3> | <h3>This picture represent the system we want to build using this promoter: a transistor like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.</h3> | ||
| Line 30: | Line 33: | ||
<h3>This chart show the response of the promoter PAM rich URS J23117Alt (BBa_K1723001) in the presence of different complexes sgRNA-dCas9-ω provided by our part collection. Specifically, we see that in presence of the complex BBa_K1723006-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is not activated. Visit our website for more informations: http://2015.igem.org/Team:EPF_Lausanne</h3> | <h3>This chart show the response of the promoter PAM rich URS J23117Alt (BBa_K1723001) in the presence of different complexes sgRNA-dCas9-ω provided by our part collection. Specifically, we see that in presence of the complex BBa_K1723006-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is not activated. Visit our website for more informations: http://2015.igem.org/Team:EPF_Lausanne</h3> | ||
<br/><br/> | <br/><br/> | ||
| − | + | ||
| − | + | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 18:33, 18 September 2015
Applications of BBa_K1723006
promoter strength was assessed using GFPmut2 reporter.
This picture represent the system we want to build using this promoter: a transistor like element in cell. The complex dCas9-sgRNA will bind on the promoter and activate or inhibit it.
Effects of dCas9-ω BBa_K1723000 on BBa_K1723005 promoter
This chart present the effects of dCas9-ω (BBa_K1723000) on the PAM rich URS J23117Alt promoter (BBa_K1723005) when provided following sgRNAs:
[A_alt] sgRNA X4 (BBa_K1723007), activating
[R2_alt] sgRNA X0 (BBa_K1723006), inhibiting
[R1_alt] sgRNA X35 (BBa_K1723008), inhibiting
In comparison with:
[B_alt] apo-dCas9-ω (=no sgRNA)
[Af_alt] no dCas9-ω, no sgRNA
This chart show the response of the promoter PAM rich URS J23117Alt (BBa_K1723001) in the presence of different complexes sgRNA-dCas9-ω provided by our part collection. Specifically, we see that in presence of the complex BBa_K1723006-dCas9-ω, the promoter PAM rich URS J23117 (BBa_K1723001) is not activated. Visit our website for more informations: http://2015.igem.org/Team:EPF_Lausanne
User Reviews
UNIQ7eb89ec0c6f640da-partinfo-00000000-QINU UNIQ7eb89ec0c6f640da-partinfo-00000001-QINU