Difference between revisions of "Part:BBa K1602058"
 
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         <h1>B0034-yagE-B0034-yjhG-B0034-yqhD</h1>
 
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         This composite represents the 2-Dehydro-3-deoxy-<small>D</small>-pentonate aldolases <i>yagE</i> the <small>D</small>-xylonic acid dehydratase <i>yjhG</i> and the aldehyde reductase <i>YqhD</i> in one plasmid. All three genes have the strong ribosome binding site B0034 before their sequence.
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         This composite represents the 2-Dehydro-3-deoxy-<small>D</small>-pentonate aldolases <html><a href="/Part:BBa_K1602011"><i>yagE</i></a></html> the <small>D</small>-xylonic acid dehydratase <html><a href="/Part:BBa_K1602012"><i>yjhG</i></a></html> and the aldehyde reductase <html><a href="/Part:BBa_K1602013"><i>YqhD</i></a></html> in one plasmid. All three genes have the strong ribosome binding site <html><a href="/Part:BBa_B0034">B0034</a></html> before their sequence.
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===<h2>Usage</h2>===
 
===<h2>Usage</h2>===
 
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Latest revision as of 22:43, 25 September 2015

B0034-yagE-B0034-yjhG-B0034-yqhD

This composite represents the 2-Dehydro-3-deoxy-D-pentonate aldolases yagE the D-xylonic acid dehydratase yjhG and the aldehyde reductase YqhD in one plasmid. All three genes have the strong ribosome binding site B0034 before their sequence.

Usage

This part is a composite of three coding genes and one strong RBS per gene (BBa_B0034).

  • yagE
  • yjhG
  • YqhD
  • RBS-B0034
https://static.igem.org/mediawiki/parts/a/aa/TU_Darmstadt_plasmid-schema_B0034-yagE-B0034-yjhG-B0034-yqhD.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1951
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2856
    Illegal AgeI site found at 3371
    Illegal AgeI site found at 3565
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1114
    Illegal SapI.rc site found at 193
    Illegal SapI.rc site found at 4028