Difference between revisions of "Part:BBa K945002:Experience"

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===Characterization by Czech Republic 2015===
 
===Characterization by Czech Republic 2015===
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The activity of CUP promoter was characterized by transforming pCUP-GFP plasmids into yeast S. cervisiae and measuring the evolution of fluorescence in time.
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[[File:K945002_Timeresponse.png|500px]]
 
[[File:K945002_Timeresponse.png|500px]]
  

Revision as of 18:16, 18 September 2015

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Characterization by Czech Republic 2015

The activity of CUP promoter was characterized by transforming pCUP-GFP plasmids into yeast S. cervisiae and measuring the evolution of fluorescence in time.

K945002 Timeresponse.png

K945002 timeactivity.png

Characterization by Tec-Monterrey EKAM iGEM 2012

The functionality of this promoter was tested by transforming Pichia pastoris with a construct containing reporter BBa_K945004 under the control of this promoter. The culture was induced with copper (II) sulfate at a concentration of 200 uM and visualized using fluorescence microscopy.

Control: Pichia pastoris visualized under the microscope.
Control: Pichia pastoris visualized with fluorescence microscopy (Zeiss filter set 18).
Transformant visualized under the microscope.
Transformant visualized with fluorescence microscopy (Zeiss filter set 18).

Applications of BBa_K945002

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