Difference between revisions of "Part:BBa K1675001"

Revision as of 17:44, 18 September 2015

NhaB; sodium-proton antiporter; membrane protein

The gene, NhaB, is the archetypal sodium/proton anti-porter in E. coli. It is crucial when NhaA’s activity is limited, such as NhaA is not sufficiently induced, or NhaA is not activated. NhaA catalyzes the exchange of 3 protons per 2 sodiums. NhaA has a high affinity for sodium and lithium. Its activity is weakly pH-dependent, which is essential for regulation of intracellular pH under alkaline conditions. Therefore, NhaA could make sure E. coli’s survival under alkaline pH, which are in desperate need of our project.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 5
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1003
Illegal AgeI site found at 1315
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1675001 short</partinfo>
-The gene, ''NhaB'', is the archetypal sodium/proton anti-porter in ''E. coli''. It is crucial when the ''NhaA'''s activity is limited, when ''NhaA'' is not sufficiently induced, and/or when ''NhaA'' is not activated.
+The gene, ''NhaB'', is the archetypal sodium/proton anti-porter in ''E. coli''. It is crucial when ''NhaA''’s activity is limited, such as ''NhaA'' is not sufficiently induced, or ''NhaA'' is not activated.
-''NhaA'' catalyzes the exchange of 3 protons per 2 sodium ions. Therefore, ''NhaA'' could make sure ''E. coli''’s survival under alkaline pH, which are in desperate need in our project.
+''NhaA'' catalyzes the exchange of 3 protons per 2 sodiums. ''NhaA'' has a high affinity for sodium and lithium. Its activity is weakly pH-dependent, which is essential for regulation of intracellular pH under alkaline conditions. Therefore, ''NhaA'' could make sure ''E. coli''’s survival under alkaline pH, which are in desperate need of our project.