Difference between revisions of "Part:BBa K1713003"
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The hybrid promoter ( Plac/ara-1 ) can be activated by the araC protein in the presence of arabinose and so that GFP produced. And the LacI protein would repress this hybrid promoter ( Plac/ara-1 ) in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG). | The hybrid promoter ( Plac/ara-1 ) can be activated by the araC protein in the presence of arabinose and so that GFP produced. And the LacI protein would repress this hybrid promoter ( Plac/ara-1 ) in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG). | ||
| + | <p>Attention: After sequencing, we found that this plasmids have two sites error on Suffix which is after the end of hybrid promoter sequence "cacaca" and the initial two bases of Suffix is changed from T-A to GAA. We can not make sure whether it will have an impact on function till now.</p> | ||
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Latest revision as of 13:12, 20 January 2016
Plac/ara-1+strong RBS+GFP(+LVA)+double terminator
The hybrid promoter ( Plac/ara-1 ) can be activated by the araC protein in the presence of arabinose and so that GFP produced. And the LacI protein would repress this hybrid promoter ( Plac/ara-1 ) in the absence of isopropyl b-D-1-thiogalactopyranoside (IPTG).
Attention: After sequencing, we found that this plasmids have two sites error on Suffix which is after the end of hybrid promoter sequence "cacaca" and the initial two bases of Suffix is changed from T-A to GAA. We can not make sure whether it will have an impact on function till now.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 28
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 772