Difference between revisions of "Part:BBa K1689019:Design"
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<partinfo>BBa_K1689019 short</partinfo>
 
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<partinfo>BBa_K1689019 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1689019 SequenceAndFeatures</partinfo>

Revision as of 17:29, 18 September 2015

delta alpha-dCas9



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4156
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 6435
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence of this fusion protein is too long, you'd better amplify it separately.


Source

E.coli

References