Difference between revisions of "Part:BBa K1689011"

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https://static.igem.org/mediawiki/parts/2/26/Peking-speculation-LacZ-2.png
 
https://static.igem.org/mediawiki/parts/2/26/Peking-speculation-LacZ-2.png
  
References:
+
References:<br/>
 
1 Mohler, W. A., & Blau, H. M. (1996). Gene expression and cell fusion analyzed by lacZ complementation in mammalian cells. Proceedings of the National Academy of Sciences, 93(22), 12423-12427.<br/>
 
1 Mohler, W. A., & Blau, H. M. (1996). Gene expression and cell fusion analyzed by lacZ complementation in mammalian cells. Proceedings of the National Academy of Sciences, 93(22), 12423-12427.<br/>
 
2 Biran, I., Klimentiy, L., Hengge-Aronis, R., Ron, E. Z., & Rishpon, J. (1999). On-line monitoring of gene expression. Microbiology, 145(8), 2129-2133.
 
2 Biran, I., Klimentiy, L., Hengge-Aronis, R., Ron, E. Z., & Rishpon, J. (1999). On-line monitoring of gene expression. Microbiology, 145(8), 2129-2133.

Revision as of 00:43, 19 September 2015

dCas9-delta alpha

dCas9 fused with Δα segment of β-glactosidase

For their ease of use, low assay time, cost-effectiveness, high sensitivity, electrochemical biosensors have emerged as an attractive method for molecular detection. Combing with our PC Reporter we may further enhance it's applicability, that is to implement point-of-care diagnostics in settings outside clinics and without the need of specialized analytical laboratories.

β-glactosidase (LacZ) is an exoglycosidase from E.coli, composing of 1024 amino acids. LacZ is a commonly used as a reporter, known for blue white screen, which catalyzes X-gal to produce blue dye; by using different substrates, for example ONPG, reaction can be measured quantitatively.

In our project, we could dissect LacZ as Δα, ∆ω segments to fuse with dCas9 to do the same as our PC Reporter.
Peking-speculation-LacZ-1.png

β-galactosidase is used to catalyze the hydrolysis of p-aminophenyl-β-D-galactopyranoside (PAPG) to p-aminophenol (PAP), which can be oxidized at an working electrode held at 220mV versus the reference electrode.
Peking-speculation-LacZ-2.png

References:
1 Mohler, W. A., & Blau, H. M. (1996). Gene expression and cell fusion analyzed by lacZ complementation in mammalian cells. Proceedings of the National Academy of Sciences, 93(22), 12423-12427.
2 Biran, I., Klimentiy, L., Hengge-Aronis, R., Ron, E. Z., & Rishpon, J. (1999). On-line monitoring of gene expression. Microbiology, 145(8), 2129-2133.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 3429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]