Difference between revisions of "Part:BBa K1590003"
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The promoter pChr is suspected to be inducible by chromate.
 
The promoter pChr is suspected to be inducible by chromate.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1590003 SequenceAndFeatures</partinfo>
  
  
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P<i>chr itself is believed to be regulated by the chromate-sensitive repressor <i>chrB</i>. This has made it a candidate for a biological sensor of chromate contamination in e.g. rivers (Branco et al.), or in milk (iGEM BIT 2013).
 
P<i>chr itself is believed to be regulated by the chromate-sensitive repressor <i>chrB</i>. This has made it a candidate for a biological sensor of chromate contamination in e.g. rivers (Branco et al.), or in milk (iGEM BIT 2013).
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K1590003 SequenceAndFeatures</partinfo>
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K1590003 parameters</partinfo>
 
<partinfo>BBa_K1590003 parameters</partinfo>

Revision as of 17:18, 18 September 2015

Chromate responsive promoter

The promoter pChr is suspected to be inducible by chromate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 135
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This promoter is found upstream of the ChrBACF - operon in Ochrobactrum tritici 5bvl1, located in the transposable element TnOtChr of 7189bp length.

Ochrobactrum tritici 5bvl1 was discovered in 2004 in a chromate contaminated wastewater treatment facility. A specific characteristic of this strain was that it could both, reduce Cr(VI) and be resistant to it at the same time. Previously characterised strains were either resistant or could reduce chromate. Over the last 10 years the group that discovered it started working out the underlying mechanisms of this set of characteristics.

The ChrBACF - operon, expressed from the Pchr - promoter led to the expression of the downstream genes chrA, chrB, chrC, and chrF. chrA was found to express and efflux pump, whereas the gene product of chrB was identified as being a chromate-inducible regulator of Pchr. Additionally, chrC and chrF were found to confer tolerance to superoxides.

Pchr itself is believed to be regulated by the chromate-sensitive repressor <i>chrB. This has made it a candidate for a biological sensor of chromate contamination in e.g. rivers (Branco et al.), or in milk (iGEM BIT 2013).


Functional Parameters