Difference between revisions of "Part:BBa K1714001"
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<p>In this page, I described the result of the activity assay of ABF-2.</p>
 
<p>In this page, I described the result of the activity assay of ABF-2.</p>
 +
 +
<p>We transformed it into <i>E. coli</i> (in this case, we used DH5α) and compared the growth curve between samples induced or not.</p>
 +
<p>Activity test for ABF-2 and thanatin</p>
 +
<p>→Comparing growth curve between the samples one is induced by IPTG and the other is not induced.</p>
 +
  <ol>
 +
  <li>Diluting the bacterial culture which is enough pre-cultured by 0.1 OD<sub>600</sub> (Optical Density in λ=600nm) in 2 mL LBC.(Lysogeny Broth liquid culture including chloramphenicol) </li>
 +
  <li>Adding 2 µL of IPTG into one and DW into the other.</li>
 +
  <li>Measuring OD<sub>600</sub> of these samples once in an hour. </li>
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  </ol>
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 +
<p>As a result, we got the graph of growth curve of DH5α and shown on below as Chart1.</p>

Revision as of 17:14, 18 September 2015

This part will be finally introduced upstream of BBa_K1714004 (PBAD-B0034-BLA-dT on 1C3) and expressed as AMPs in Co-expression system. In this page, I described the result of the activity assay of ABF-2.

In this page, I described the result of the activity assay of ABF-2.

We transformed it into E. coli (in this case, we used DH5α) and compared the growth curve between samples induced or not.

Activity test for ABF-2 and thanatin

→Comparing growth curve between the samples one is induced by IPTG and the other is not induced.

  1. Diluting the bacterial culture which is enough pre-cultured by 0.1 OD600 (Optical Density in λ=600nm) in 2 mL LBC.(Lysogeny Broth liquid culture including chloramphenicol)
  2. Adding 2 µL of IPTG into one and DW into the other.
  3. Measuring OD600 of these samples once in an hour.

As a result, we got the graph of growth curve of DH5α and shown on below as Chart1.