Difference between revisions of "Part:BBa K1819004"

Revision as of 16:48, 18 September 2015

GFP+TetR+pTetR+mRFP1

This construction gives the possibility of adding the most suitable promoter in order to regulate GFP transcription and any genetic sequence inserted after RBS and before TetR. In the absence of tetracycline bound in the tetR site, the TetR promoter (BBa_R0040) transcripts the RFP (or the gene located in the region corresponding to RFP). You can see some results on iGEM team Brasil-USP's wiki page using a Lactose promoter to control GFP and RFP expression.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2211
Illegal AgeI site found at 2323
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 644

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1819004 short</partinfo>
+<html>
+<p>
 This construction gives the possibility of adding the most suitable promoter in order to regulate GFP transcription and any genetic sequence inserted after RBS and before TetR. In the absence of tetracycline bound in the tetR site, the TetR promoter  (BBa_R0040) transcripts the RFP (or the gene located in the region corresponding to RFP).
-You can see some results on <a href ="">iGEM team Brasil-USP's wiki page</a> using a Lactose promoter to control GFP and RFP expression.
+You can see some results on <a href ="http://2015.igem.org/Team:Brasil-USP">iGEM team Brasil-USP's wiki page</a> using a Lactose promoter to control GFP and RFP expression.
+</p>
+</html>
 <!-- Add more about the biology of this part here