Difference between revisions of "Part:BBa K1642011:Design"
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<partinfo>BBa_K1642011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1642011 SequenceAndFeatures</partinfo>
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[[File:SJTUB wb Pdark-HR.jpeg|200px|thumb|left|alt text]]
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[[File:SJTUB wb Pdark-HR.tif|200px|thumb|left|alt text]]
  
 
===Design Notes===
 
===Design Notes===

Revision as of 16:35, 18 September 2015

Expression of Halorhodopsin under the control of Pdrak


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 611
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
    Illegal NgoMIV site found at 783
    Illegal NgoMIV site found at 942
    Illegal NgoMIV site found at 1263
    Illegal AgeI site found at 1134
  • 1000
    COMPATIBLE WITH RFC[1000]
File:SJTUB wb Pdark-HR.tif
alt text

Design Notes

Pdark(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Control_System)

HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Transport_Module)

Pdark-HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Design)

Source

Overlap PCR from basic parts Up(BBa_K1642005), Pdark(BBa_K1026009), RBS(BBa_K1642004), HR(BBa_K1642001), Terminator(BBa_B0012), PcpcB-Kana(BBa_K1642003), Down(BBa_K1642006).