Difference between revisions of "Part:BBa K1796001"

Revision as of 16:49, 18 September 2015

promoter of Paenibacillus sp. WLY78 nif gene cluster

The promoter of Paenibacillus sp. WLY78 nif gene cluster, We named it Pnif. Pnif is a σ70-dependent promoter located upstream of nifB. Pnif is in control of all the gene clusters of nitrogen fixation genome. Confirmation of nif promoter We standardized the nif promoter of Paenibacillus sp. WLY78, and linked it to the upstream of 3 reporter genes (RFP reporter: BBa_K1357010, YFP reporter: BBa_E0430;amilCP reporter: BBa_K1357009) to confirm the reliability of this promoter. The results are shown in Figure 2. A B C

Fig.4. Confirmation of nif promoter of Paenibacillus sp. WLY78. (A) Pnif+RFP reporter; (B) Pnif+YFP reporter;(C)Pnif+amilCP reporter.

The results confirmed the reliability of Pnif. The initiation of Pnif does not require inducement. Thus it is a effective constitutive promoter.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

Relative promoter strength estimates

Reference	Strength
BBa_K1796001	13
BBa_K176011	67

type	constitutive promoter

@@ Line 23: / Line 23: @@
-<!-- Uncomment this to enable Functional Parameter display
+<!-- Uncomment this to enable Functional Parameter display-->
 ===Functional Parameters===
 <p>Relative promoter strength estimates</p>
-<p>123456123456</p>
+{|border="1"
-2222222222222222
+!Reference
+!Strength
+|-
+|<partinfo>BBa_K1796001</partinfo>
+|13
+|-
+|<partinfo>BBa_K176011</partinfo>
+|67
+|}
 <partinfo>BBa_K1796001 parameters</partinfo>
 <!-- -->
+==SCU_China's MEASUREMENT==
+https://static.igem.org/mediawiki/2015/thumb/9/98/Comparison_of_Pnif_and_Pd.jpeg/800px-Comparison_of_Pnif_and_Pd.jpeg