Difference between revisions of "Part:BBa K1157006:Experience"

Revision as of 15:04, 18 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1157006

We used flow cytometry to test the individual fluorescence expression of our biobrick from 0 to 4 hours and in different concentration(10^-3, 10^-5,10^-7).

User Reviews

UNIQ6b42a66f6dd970a9-partinfo-00000000-QINU

•

iGEM_Stockholm_2015

Method: Promoter, RBS, RhlI, and double terminator BioBricks were transformed in TOP10 E. coli using the transformation protocol and were supposed to be assembled using the 3A assembly protocol.

Results: During the initial lab weeks there were some trouble with transformation, later addressed to not using KCM buffer. The BBa_K082035 was ordered from IDT to speed up the process after repeated failed transformations and transformation troubleshooting were done in parallel.

Conclusion: BBa_K082035 was ordered instead of cloned.

;

@@ Line 25: / Line 25: @@
 <I>iGEM_Stockholm_2015</I>
 |width='60%' valign='top'|
-'''Method:''' Promoter, RBS, RhlI, and double terminator BioBricks were transformed in TOP10 E. coli using the transformation protocol and were supposed to be assembled using the 3A assembly protocol.
+'''Method:''' Promoter, RBS, RhlI, and double terminator BioBricks were transformed in TOP10 ''E. coli'' using the [https://static.igem.org/mediawiki/2015/c/cc/STHLM_Transformation_protocol.pdf transformation protocol] and were supposed to be assembled using the 3A assembly protocol.
 '''Results:''' During the initial lab weeks there were some trouble with transformation, later addressed to not using KCM buffer. The BBa_K082035 was ordered from IDT to speed up the process after repeated failed transformations and transformation troubleshooting were done in parallel.