Difference between revisions of "Part:BBa K1593210"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | == T7 == | ||
| + | Information of T7:see <html><a href="https://parts.igem.org/Part:BBa_I712074" target="_blank">BBa_I712074</a> | ||
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| + | == OprF == | ||
| + | Informtion of OprF:see <html><a href="https://parts.igem.org/Part:BBa_K1593209" target="_blank">BBa_K1593209</a> | ||
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| + | == Growth Characterization == | ||
| + | This is in order to demonstrate that the overexpression of porin won't severely affect the growth of bacteria, at least it won't significantly inhibit bacteria growth and become as kill switch. | ||
| + | [[File:1.jpg]] | ||
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Revision as of 14:33, 18 September 2015
T7-OprF
Using T7 strong promoter to express OprF, derived from Pseudomonus areuginosa, would strongly improve the bacterial permeability.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 654
- 1000COMPATIBLE WITH RFC[1000]