Difference between revisions of "Part:BBa K1593210"
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Using T7 strong promoter to express OprF, derived from Pseudomonus areuginosa, would strongly improve the bacterial permeability. | Using T7 strong promoter to express OprF, derived from Pseudomonus areuginosa, would strongly improve the bacterial permeability. | ||
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== T7 == | == T7 == | ||
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Later on, we will characterize the basic permeability capability of *E. coli* BL21 with T7-OprF ([BBa_K1593210](https://parts.igem.org/Part:BBa_K1593210)) and *E. coli* BL21 with T7-SCVE, ([BBa_K1593667](https://parts.igem.org/Part:BBa_K1593667)). | Later on, we will characterize the basic permeability capability of *E. coli* BL21 with T7-OprF ([BBa_K1593210](https://parts.igem.org/Part:BBa_K1593210)) and *E. coli* BL21 with T7-SCVE, ([BBa_K1593667](https://parts.igem.org/Part:BBa_K1593667)). | ||
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Revision as of 14:13, 18 September 2015
T7-OprF
Using T7 strong promoter to express OprF, derived from Pseudomonus areuginosa, would strongly improve the bacterial permeability.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 654
- 1000COMPATIBLE WITH RFC[1000]