Difference between revisions of "Part:BBa K1859024"
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<p> | <p> | ||
| − | We designed this generator [ | + | We designed this generator [BBa_K1859024] to improve circular efficiency of mRNA including His-RFP sequence. |
</p> | </p> | ||
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859004" > [BBa_K1859004] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859004" > [BBa_K1859004] </a> | ||
), His-RFP without stop codon | ), His-RFP without stop codon | ||
| − | <a href= "https://parts.igem.org/wiki/index.php?title=Part: | + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332002" >[BBa_K1332002] </a> |
, Lacl | , Lacl | ||
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a> | ||
Revision as of 09:22, 22 September 2015
mRNA circularize efficiently generator (inside complementarityⅠ)
We designed this generator [BBa_K1859024] to improve circular efficiency of mRNA including His-RFP sequence.
The generator is comprised of Circular parts combined the linker( [BBa_K1859003] and [BBa_K1859004] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .
Linkers designed to adjust folding of protein are in [BBa_K1859003] and [BBa_K1859004] . RNA sequences of these linkers bind complementary with each other in circular mRNA.
We found that circular efficiency of [BBa_1859024] was the third, when we compared with amount of circular mRNA made by these generators [BBa_1332011] , [BBa_1859024], [BBa_K1859025] , [BBa_K1859026] .