Difference between revisions of "Part:BBa K1789014"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1789014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1789014 SequenceAndFeatures</partinfo>
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==Experimental Validation==
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===Sequencing===
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This part is sequenced as correct after construction.
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===Enzyme digestion test ===
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A double-enzyme digestion test was implemented to verify this part.
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[[File:SCAF2+Ter酶切.jpg|250px|]]
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The length of the digested DNA fits the design.
  
  

Revision as of 14:28, 18 September 2015

SCAF2+Ter

It is the combination of scaffold system and termination.

Usage and Biology

Usage: This part is SCAF2 which is associated with a terminator. SCAF2 is used to bind TALE1 and TALE3 protein to make them fixed on the DNA. We create this part to make it more convenient every time we are required to add a scaffold.

Biology: SCAF2 is a DNA scaffold which can bind TALE protein specifically to achieve the fixing of protein or enzyme. This part is scaf2 with a terminator after it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
    Illegal AgeI site found at 180
    Illegal AgeI site found at 210
    Illegal AgeI site found at 240
    Illegal AgeI site found at 270
    Illegal AgeI site found at 300
    Illegal AgeI site found at 330
  • 1000
    COMPATIBLE WITH RFC[1000]

Experimental Validation

Sequencing

This part is sequenced as correct after construction.

Enzyme digestion test

A double-enzyme digestion test was implemented to verify this part.

SCAF2+Ter酶切.jpg

The length of the digested DNA fits the design.


Functional Parameters

CHIp

Enzyme digestion test /