Difference between revisions of "Part:BBa K1688004"
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| − | Figure 1. dTomato expressed in E. coli DH5-alpha with an inducible promoter. | + | Figure 1. dTomato expressed in E. coli DH5-alpha with an |
| + | inducible promoter. | ||
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The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use – especially in self-made fluorometry tests – because the excitation wavelengths and the emission wavelengths don't overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. Also, its emission peak is at 581 nm and its 50% emission at 629 nm (Figure 1). Characterization has been made with the Anderson promoters BBa_J23101 (construct BBa_K1688010) and BBa_J23110 (construct BBa_K1688011) | The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use – especially in self-made fluorometry tests – because the excitation wavelengths and the emission wavelengths don't overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. Also, its emission peak is at 581 nm and its 50% emission at 629 nm (Figure 1). Characterization has been made with the Anderson promoters BBa_J23101 (construct BBa_K1688010) and BBa_J23110 (construct BBa_K1688011) | ||
Revision as of 14:02, 18 September 2015
dTomato with RBS
Figure 1. dTomato expressed in E. coli DH5-alpha with an inducible promoter.
The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use – especially in self-made fluorometry tests – because the excitation wavelengths and the emission wavelengths don't overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. Also, its emission peak is at 581 nm and its 50% emission at 629 nm (Figure 1). Characterization has been made with the Anderson promoters BBa_J23101 (construct BBa_K1688010) and BBa_J23110 (construct BBa_K1688011)
Figure 2. Excitation (blue curve) and emission (red curve) spectra for the dTomato fluorescent protein (the graph is designed with the following tool: https://www.chroma.com/spectra-viewer)