Difference between revisions of "Part:BBa K1680026"

 
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<partinfo>BBa_K1680026 short</partinfo>
 
<partinfo>BBa_K1680026 short</partinfo>
  
''E. coli'' expression plasmid. Overexpression is IPTG inducible in the RFC25 Standard. It also encodes for a 6xHis-tag with a Thromin cleavage site between coding region and 6xHis-tag, so that the His-tag can easily be cleaved off. Vector can be linearized with PstI/XmaI, and parts can be cloned into it with PstI/NgoMIV.   
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''E. coli'' expression plasmid. Overexpression is IPTG inducible in the RFC25 Standard. It also encodes for a 6xHis-tag with a Thrombin cleavage site between coding region and 6xHis-tag, so that the His-tag can easily be cleaved off. Vector can be linearized with PstI/XmaI, and parts can be cloned into it with PstI/NgoMIV.   
 
Ampicillin resistance.
 
Ampicillin resistance.
  

Latest revision as of 00:28, 26 September 2015

pETUE

E. coli expression plasmid. Overexpression is IPTG inducible in the RFC25 Standard. It also encodes for a 6xHis-tag with a Thrombin cleavage site between coding region and 6xHis-tag, so that the His-tag can easily be cleaved off. Vector can be linearized with PstI/XmaI, and parts can be cloned into it with PstI/NgoMIV. Ampicillin resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 133
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal PstI site found at 133
    Illegal NgoMIV site found at 892
    Illegal NgoMIV site found at 3053
    Illegal NgoMIV site found at 3213
    Illegal AgeI site found at 3270
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 374
    Illegal BsaI.rc site found at 1477