Difference between revisions of "Part:BBa K1859027"
| Line 28: | Line 28: | ||
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859004" > [BBa_K1859004] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859004" > [BBa_K1859004] </a> | ||
), His-RFP without stop codon | ), His-RFP without stop codon | ||
| − | <a href= "https://parts.igem.org/wiki/index.php?title=Part: | + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332002" >[BBa_K1332002] </a> |
, Lacl | , Lacl | ||
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a> | ||
Revision as of 09:26, 22 September 2015
Histidine tag and RFP linker [GSGSGS] semi-permanent generator
We designed this generator[K1859027] to express long-chain protein with keeping functionality of the protein, giving the red fluorescence.
In the study of iGEM Gifu 2014, we made long-chain protein from Circular mRNA, but it lost functionality. It is thought that folding of the protein failed for a cause．
Therefore we designed some linkers to fix the folding of the protein. Then, we combined circular parts, the 3'side of the intron [BBa_K1332005] and the 5'side of the intron [BBa_K1332003] , with those linkers and made them parts. The generator is comprised of Circular parts combined the linker( [BBa_K1859001] and [BBa_K1859004] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .
E. coli introduced the generator synthesized long-chain protein,but the protein had no fluorescence.