Difference between revisions of "Part:BBa K1713015:Design"
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===References=== | ===References=== | ||
Revision as of 13:16, 18 September 2015
luxpR+B0034+LuxI(-LVA)+B0032+AiiA(-LVA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 718
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 850
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The promote LuxPR is regulated by the concentration of AHL (N-Acyl homoserine lactone). Aiia is a kind of enzyme could hydrolyze AHL. LuxI is a kind of enzyme actives the synthetic of AHL. LuxI's RBS is B0034, Aiia's RBS is B0032. LVA is a degradation tag.
Source
All the basic parts come from iGEM distribution.