Difference between revisions of "Part:BBa K1859026"
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_1332011" > [BBa_18559025]</a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_1332011" > [BBa_18559025]</a> | ||
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Revision as of 13:05, 18 September 2015
mRNA circularize efficiently generator (outside complementarity)
We designed this generator [K1859026] to improve circular efficiency of mRNA including His-RFP sequence.
First, we improved Circular Parts of iGEM2014,the 5´side of the intron [BBa_K1332003] and the 3´side of the intron [BBa_K1332005] , and made BBa_K1859015 and BBa_K1859016. Circular parts of iGEM2014 were cloned only sequence of ribozyme involved the splicing from td intron in T4 phage. However, BBa_K1859015 and BBa_K1859016 were cloned the sequence except the ribozyme extra in addition to [BBa_K1332003] and [BBa_K1332005] .
The generator is comprised of Circular parts improved to become complementary ( [BBa_K1859015] and [BBa_K1859016] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .
Tne RNA made by this generator [BBa_1859026] was designed to bind outside of circular mRNA complementary.
We found that circular efficiency of [BBa_1859026] was the best, when we compared with amount of circular mRNA made by these generators [BBa_1332011] , [BBa_1859024] , [BBa_18559025] ,[BBa_1859026].