Difference between revisions of "Part:BBa K1736300:Design"

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===References===
 
===References===
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<sup>1</sup> Glascock CB. et al. Using chromosomal lacIQ1 to control expression of genes on high-copy-number plasmids in Escherichia coli. Gene. 1998 Nov 26;223(1-2):221-31.

Latest revision as of 13:00, 18 September 2015


LacI-LacIQ1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PLacIQ1 promoter is placed upstream of RBS and LacI sequence. It is a strong consitutive promoter 1. The RBS is from wild type E.coli.

Source

Bacterial LacI and PLacIQ1 strong constitutive promoter developed by Glascock et al. 1.

References

1 Glascock CB. et al. Using chromosomal lacIQ1 to control expression of genes on high-copy-number plasmids in Escherichia coli. Gene. 1998 Nov 26;223(1-2):221-31.