Difference between revisions of "Part:BBa K1807000:Design"
Line 1: Line 1:
 +
<html>
 +
<body>
 +
<img src="https://static.igem.org/mediawiki/2015/thumb/a/a8/Padaptyork.png/800px-Padaptyork.png" style="width:75%; height=auto">
 +
</body>
 +
</html>
 +
 +
Above is a diagram of our Adapter BioBrick in the pSB1C3 vector.
 +
Our design incorporates a SmaI restriction digestion step and subsequent use of the cut Adapter as a vector in a Gibson Assembly Reaction or any other assembly method based on flanking overlapping sequences (as depicted below).
 +
 
<html>
 
<html>
 
<body>
 
<body>
Line 4: Line 13:
 
</body>
 
</body>
 
</html>
 
</html>
 +
  
 
BBa_K1807000 was designed as a blue-white screening device that would also be easily used in Gibson Assembly.
 
BBa_K1807000 was designed as a blue-white screening device that would also be easily used in Gibson Assembly.

Revision as of 15:23, 18 September 2015

Above is a diagram of our Adapter BioBrick in the pSB1C3 vector. Our design incorporates a SmaI restriction digestion step and subsequent use of the cut Adapter as a vector in a Gibson Assembly Reaction or any other assembly method based on flanking overlapping sequences (as depicted below).


BBa_K1807000 was designed as a blue-white screening device that would also be easily used in Gibson Assembly. Surrounding the lacZ alpha coding sequence are two SmaI restriction sites (CCC/GGG). SmaI is a blunt-end endonuclease- we used it to simulatenously linearize our vector and remove the lacZ alpha coding sequence. Our BioBricks contained overhangs that make them compatible with the SmaI-digested BBa_K1807000.

The Overhang Sequences are as follows:

BBa_K1807000 Assembly 5'end Overhang (complementary to pSB1C3): cgctaaggatgatttctgGAATTCGCGGCCGCTTCTAGAG

BBa_K1807000 Assembly 3'end Overhang (complementary to pSB1C3): TACTAGTAGCGGCCGCTGCAGtccggcaaaaaagggcaag


The subparts of the device are as follows: BBa_R0011, BBa_B0034, BBa_E0038, BBa_B0015.