Difference between revisions of "Part:BBa K1813063"

Revision as of 00:48, 19 September 2015

ptac pelB AgCPR pelb CYP2D6

With ptac powering the transcription of BBa_K1813053 and BBa_K1813055, this part allows the co-localization of large amounts of simultaneously expressed CYP2D6 ( BBa_K1813005) and A. gambiae NADPH P450 reductase ( BBa_K1813010) to the bacterial periplasm.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2783
Illegal BamHI site found at 626
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 141
Illegal NgoMIV site found at 2448
Illegal NgoMIV site found at 3373
Illegal AgeI site found at 424
Illegal AgeI site found at 1606
Illegal AgeI site found at 1762
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2122

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1813063 short</partinfo>
-ptac pelB AgCPR pelb HUMCYPDB1
+ptac pelB AgCPR pelb CYP2D6
-With ptac powering the transcription of <html><b> <a href="https://parts.igem.org/Part:BBa_K1813053">BBa_K1813053</a></b></html> and <html><b> <a href="https://parts.igem.org/Part:BBa_K1813055">BBa_K1813055</a></b></html>, this part allows the co-localization of large amounts of simultaneously expressed HUMCYPDB1 (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</a></b></html>) and <i>A. gambiae</i> NADPH p450 reductase (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813010">BBa_K1813010</a></b></html>) to the bacterial periplasm.
+With ptac powering the transcription of <html><b> <a href="https://parts.igem.org/Part:BBa_K1813053">BBa_K1813053</a></b></html> and <html><b> <a href="https://parts.igem.org/Part:BBa_K1813055">BBa_K1813055</a></b></html>, this part allows the co-localization of large amounts of simultaneously expressed CYP2D6 (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</a></b></html>) and <i>A. gambiae</i> NADPH P450 reductase (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813010">BBa_K1813010</a></b></html>) to the bacterial periplasm.