Difference between revisions of "Part:BBa K1789022"
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In this device, the TALE1-fused IAAM and the TALE2-fused IAAH are incorporated in the E.coli due to the specific binding of TALE proteins and scaffold2, which could efficiently guide the enzymes around the DNA scaffolds, to enrich the local enzyme concentration, and promote the rate of reaction. | In this device, the TALE1-fused IAAM and the TALE2-fused IAAH are incorporated in the E.coli due to the specific binding of TALE proteins and scaffold2, which could efficiently guide the enzymes around the DNA scaffolds, to enrich the local enzyme concentration, and promote the rate of reaction. | ||
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==Sequence and Features== | ==Sequence and Features== |
Revision as of 11:41, 18 September 2015
IAA(+/+)S2
This is a device which contains the sequence of the recombination of TALE1 and IAAM, the recombination of TALE2 and IAAH and scaffold 2. Through this device, we can test and verify that our scaffold can strengthen the function of IAA.
Usage and Biology
Indole-3-acetic acid (IAA), also known as auxin, is a plant hormone, which can promote growth and protect the soil from erosion. IAA can be synthesized through IAM pathway, originated from Pseudomonas savastanoi. Two important enzymes are contained in this pathway, AKA IaaM and IaaH.
In this device, the TALE1-fused IAAM and the TALE2-fused IAAH are incorporated in the E.coli due to the specific binding of TALE proteins and scaffold2, which could efficiently guide the enzymes around the DNA scaffolds, to enrich the local enzyme concentration, and promote the rate of reaction.
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2724
Illegal BamHI site found at 2311
Illegal BamHI site found at 3669
Illegal BamHI site found at 6072 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1304
Illegal BsaI.rc site found at 5166
Illegal BsaI.rc site found at 5370