Difference between revisions of "Part:BBa K1761003:Experience"

Revision as of 20:29, 18 September 2015

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Applications of BBa_K1761003

Application TU Eindhoven 2015

mNeonGreen is used as an intracellulair signaling domain. In combination with the Luciferase NanoLuc a working BRET (Bioluminescence Resonance Energy Transfer) couple is formed. For a broader description on how we used this part, take a look at part BBa_K1761001 [1], because it also contains this part.

Another application of mNeonGreen as signaling domain, is by using it with another fluorophore (for example mTurquoise2) and preform a FRET (Förster Resonance Energy Transfer). We as a team preferred to use BRET, because then substrate needs to be added, instead of using a laser for excitation, which can lead to more background noise.

mNeonGreen was also expressed with the spromotor J23101 [2] for quantification. This promotor was also used by our team for the 2015 InterLab Study, but then with the GFP protein, part I13504 [3].

User Reviews

UNIQ383e9ce8c27e76cd-partinfo-00000000-QINU UNIQ383e9ce8c27e76cd-partinfo-00000001-QINU

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 == Application TU Eindhoven 2015 ==
-mNeonGreen is used as an intracellulair signaling domain. In combination with the Luciferase NanoLuc a BRET (Bioluminescence Resonance Energy Transfer) couple is formed. For a broader description on how we used this part, take a look at part BBa_K1761001 [https://parts.igem.org/Part:BBa_K1761001].
+mNeonGreen is used as an intracellulair signaling domain. In combination with the Luciferase NanoLuc a working BRET (Bioluminescence Resonance Energy Transfer) couple is formed. For a broader description on how we used this part, take a look at part BBa_K1761001 [https://parts.igem.org/Part:BBa_K1761001], because it also contains this part.
+Another application of mNeonGreen as signaling domain, is by using it with another fluorophore (for example mTurquoise2) and preform a FRET (Förster Resonance Energy Transfer). We as a team preferred to use BRET, because then substrate needs to be added, instead of using a laser for excitation, which can lead to more background noise.
+mNeonGreen was also expressed with the spromotor J23101 [https://parts.igem.org/Part:BBa_J23101] for quantification. This promotor was also used by our team for the 2015 InterLab Study, but then with the GFP protein, part I13504 [https://parts.igem.org/Part:BBa_I13504].