Difference between revisions of "Part:BBa K1638039"
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Revision as of 10:19, 18 September 2015
Hfq fused to T25 domain of CyaA with cAMP-induced RFP reporter
The Hfq RNA-binding protein fused to the T25-domain of the adenylate cyclase, CyaA, from Bordetella pertussis. This construct can be used in the two-hybrid screening of protein-protein interactions between CsrA and another x protein fused to the T18 domain. If a library of peptide aptamers is fused to the T18-domain, one can screen for specific peptide aptamers against CsrA.
Click in on the following parts to dig deeper into the two-hybrid screening and learn how to construct your own experiment for detection of protein-protein interactions:
BBa_K1638033
BBa_K1638005
If you want to do a screening for peptide aptamers we recommend the following parts:
BBa_K1638018
BBa_K1638035
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1851
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 712
Illegal AgeI site found at 824 - 1000COMPATIBLE WITH RFC[1000]