Difference between revisions of "Part:BBa K1813055"
 
Line 2: Line 2:
 
<partinfo>BBa_K1813055 short</partinfo>
 
<partinfo>BBa_K1813055 short</partinfo>
  
−
Coding sequence of HUMCYPDB1 (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</a></b></html>) prefixed by the pelB localization signal.
+
Coding sequence of CYP2D6 (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K1813005</a></b></html>) prefixed by the pelB localization signal.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:46, 19 September 2015

pelB CYP2D6

Coding sequence of CYP2D6 ( BBa_K1813005) prefixed by the pelB localization signal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

The pelB localization signal targets a protein it is bound to for transport to the bacterial periplasm, upon which it is cleaved off. This action can bolster the stability of some proteins, due to the relatively stronger reducing conditions prevalent in the bacterial periplasm.
This part is used in BBa_K1813056