Difference between revisions of "Part:BBa K1789010:Design"
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| − | This part is built and standardized by ourselves using | + | This part is built and standardized by ourselves using Golden Gate technique. |
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Latest revision as of 02:00, 19 September 2015
TALE3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2083
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 565
Illegal BsaI.rc site found at 973
Illegal BsaI.rc site found at 1279
Design Notes
This part is designed based on the REVERSED sequence of BM2 in our project.
We finished the construction of this part using the Golden Gate TALEN and TAL Effector Kit 2.0 by Addgene. The vector was edited (adding corresponding cutting sites to be specific) to meet the RFC10 bio-brick standard.
Fig. 1 The Sequence of TALE3 cDNA and deduced amino acids
The protocol can be reached in our wiki's protocol page.
Source
This part is built and standardized by ourselves using Golden Gate technique.