Difference between revisions of "Part:BBa K1813025"
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<partinfo>BBa_K1813025 short</partinfo> | <partinfo>BBa_K1813025 short</partinfo> | ||
− | A construct used to achieve the bacterial expression of CYP6G1 (<html><b><a href="https://parts.igem.org/BBa_K1813004 | + | A construct used to achieve the bacterial expression of CYP6G1 (<html><b><a href="https://parts.igem.org/Part:BBa_K1813004>BBa_K1813004</b></a></html>) under the regulative authority of the LacI repressor. |
Revision as of 08:09, 18 September 2015
CYP6G1 Expression Cassette with LacI Reversed
A construct used to achieve the bacterial expression of CYP6G1 (Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 105
Illegal BglII site found at 1737 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2327
- 1000COMPATIBLE WITH RFC[1000]
Background
Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of CYP6G1 without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.