Difference between revisions of "Part:BBa K1813024"
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− | Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of | + | Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of HUMCYPDB1 without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host. |
Revision as of 08:12, 18 September 2015
CYP2D6 Expression Cassette with LacI Reversed
A construct used to achieve the bacterial expression of HUMCYPDB1 (BBa_K1813005) under regulative authority of the LacI repressor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 105
Illegal BglII site found at 1737 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2327
- 1000COMPATIBLE WITH RFC[1000]
Background
Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of HUMCYPDB1 without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.