Difference between revisions of "Part:BBa K1813013"
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<partinfo>BBa_K1813013 short</partinfo> | <partinfo>BBa_K1813013 short</partinfo> | ||
− | A construct used to achieve the overexpression of HUMCYPDB1/CYP2D6 ( <html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K11813005</b></a></html> in a bacterial host. | + | A construct used to achieve the overexpression of HUMCYPDB1/CYP2D6 (<html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K11813005</b></a></html>) in a bacterial host. |
Structure: ptac:: HUMCYPDB1:: term. | Structure: ptac:: HUMCYPDB1:: term. | ||
Revision as of 00:30, 19 September 2015
CYP2D6 Expression Cassette
A construct used to achieve the overexpression of HUMCYPDB1/CYP2D6 (BBa_K11813005) in a bacterial host. Structure: ptac:: HUMCYPDB1:: term.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 410
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1000
- 1000COMPATIBLE WITH RFC[1000]
Background
Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of HUMCYPDB1 by a bacterial organism harboring this construct.
This construct was assembled by Michael VanInsberghe, and is codon optimized for E. coli .
It is used in BBa_K1813024 and BBa_K1813032.