Difference between revisions of "Part:BBa K1813020"
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<partinfo>BBa_K1813020 parameters</partinfo>
 
<partinfo>BBa_K1813020 parameters</partinfo>
 
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<h2>Background of <i>LacI Ptac NicD Term</i></h2>
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<h2>Background of <i>LacI Ptac NicC Term</i></h2>
 
   
 
   
 
<h4>Part Description:</h4>
 
<h4>Part Description:</h4>
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<h4>Design and Acquisition</h4>
 
<h4>Design and Acquisition</h4>
 
<p>  
 
<p>  
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After synthesizing a codon-optimized NicD, we used standard assembly to created a composite part composed of NicD driven by the Ptac promoter (BBa_K1813037) and flanked by a double terminator (BBa_B0014). The tac promoter contains a lac operator sequence that can be bound by LacI, the lac repressor protein, allowing inducible expression by Isopropyl β-D-1-thiogalactopyranoside (IPTG). Our NicD expression cassette was assembled behind a lacI cassette (BBa_K1813019) to give us the ability to control the expression of NicD. All nicD constructs are contained within standard pSB1C3 vectors.</p>
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After synthesizing a codon-optimized NicC, we used standard assembly to created a composite part composed of NicC driven by the Ptac promoter (BBa_K1813037) and flanked by a double terminator (BBa_B0014). The tac promoter contains a lac operator sequence that can be bound by LacI, the lac repressor protein, allowing inducible expression by Isopropyl β-D-1-thiogalactopyranoside (IPTG). Our NicC expression cassette was assembled behind a lacI cassette (BBa_K1813019) to give us the ability to control the expression of NicC. All nicC constructs are contained within standard pSB1C3 vectors.</p>
  
 
<h4>Experience</h4>
 
<h4>Experience</h4>
 
<p><p>
 
<p><p>

Revision as of 05:30, 18 September 2015

nicC Expression Cassette with LacI Reversed

LacI Reversed nicC


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1658
  • 1000
    COMPATIBLE WITH RFC[1000]


Background of LacI Ptac NicC Term

Part Description:


Part Origin

where part originated and Genus species more words if needed

Part Function

part functionstuff

Design and Acquisition

After synthesizing a codon-optimized NicC, we used standard assembly to created a composite part composed of NicC driven by the Ptac promoter (BBa_K1813037) and flanked by a double terminator (BBa_B0014). The tac promoter contains a lac operator sequence that can be bound by LacI, the lac repressor protein, allowing inducible expression by Isopropyl β-D-1-thiogalactopyranoside (IPTG). Our NicC expression cassette was assembled behind a lacI cassette (BBa_K1813019) to give us the ability to control the expression of NicC. All nicC constructs are contained within standard pSB1C3 vectors.

Experience

<p>