Difference between revisions of "Part:BBa K1640022"
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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<partinfo>BBa_K1640022 parameters</partinfo> | <partinfo>BBa_K1640022 parameters</partinfo> | ||
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+ | ===Overview=== | ||
+ | |||
+ | This biobrick contains a gene from the photosystem II complex of ''Chlamydomonas reinhardtii'' - psbP, with RBS included. | ||
+ | |||
+ | This part was designed in conjunction with <html><a href="https://parts.igem.org/Part:BBa_K1640012">BBa_K1640012</a></html> and <html><a href="https://parts.igem.org/Part:BBa_K1640023">BBa_K1640023</a></html>, together they form operon 5 in our set of photosystem II operons:<br><br> | ||
+ | |||
+ | <html><center><img src="https://static.igem.org/mediawiki/parts/thumb/a/a1/Macquarie_PS2_diagram.png/800px-Macquarie_PS2_diagram.png" width="450px" alt="PSII diagram"></center></html> | ||
+ | |||
+ | ===Biology & Literature=== | ||
+ | |||
+ | The psbP gene that comprises this part transcribes a protein that optimizes the availability of Ca2+ and Cl- cofactors in the Oxygen Evolving Complex in PSII to maintain the active Manganese cluster. | ||
+ | |||
+ | ===Protein information=== | ||
+ | |||
+ | '''psbP''' | ||
+ | |||
+ | mass: 23.17kDa | ||
+ | |||
+ | sequence:<br> | ||
+ | MASGSDVSRRAALAGFAGAAALVSSSPANAAYGDSANVFGKVTNKSGFVPYAGDGFALLLPAKWNPSKENDF | ||
+ | PGVILRYEDNFDAVNNLVVIAQDTDKKAIADFGSQDKFLESVSYLLGKQAYSGETQSEGGFAPNRVSAASLL | ||
+ | DVSTTTDKKGKTYYKYELLVRSADGDEGGRHQLIGATVGSDNKLYIIKIQIGDKRWFKGAKKEAMGAFDSFT | ||
+ | VV* | ||
+ | |||
+ | |||
+ | |||
+ | ===References=== |
Revision as of 10:02, 18 September 2015
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 537
- 1000COMPATIBLE WITH RFC[1000]
Overview
This biobrick contains a gene from the photosystem II complex of Chlamydomonas reinhardtii - psbP, with RBS included.
This part was designed in conjunction with BBa_K1640012 and BBa_K1640023, together they form operon 5 in our set of photosystem II operons:
Biology & Literature
The psbP gene that comprises this part transcribes a protein that optimizes the availability of Ca2+ and Cl- cofactors in the Oxygen Evolving Complex in PSII to maintain the active Manganese cluster.
Protein information
psbP
mass: 23.17kDa
sequence:
MASGSDVSRRAALAGFAGAAALVSSSPANAAYGDSANVFGKVTNKSGFVPYAGDGFALLLPAKWNPSKENDF
PGVILRYEDNFDAVNNLVVIAQDTDKKAIADFGSQDKFLESVSYLLGKQAYSGETQSEGGFAPNRVSAASLL
DVSTTTDKKGKTYYKYELLVRSADGDEGGRHQLIGATVGSDNKLYIIKIQIGDKRWFKGAKKEAMGAFDSFT
VV*