Difference between revisions of "Part:BBa K1628005"

Revision as of 06:03, 18 September 2015

A2up

Promoter A2up is an artificially synthesized promoter. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to our promoter strength assay in B. amyloliquefaciens NK-1 (showed in Figure 1), A2up is a very strong promoter in B. amyloliquefaciens NK-1.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1628005 short</partinfo>
-Promoter A2up is an artificially synthesized promoter. We have validate this part through promoter strength assay using ''bgaB'' (encoding β-galatosidase downstream of the multiple cloning sites) as reporter genes. According to our promoter strength assay in ''B. amyloliquefaciens'' NK-1 (showed in Figure 1), A2up is a very strong promoter in ''B. amyloliquefaciens'' NK-1.
+Promoter A2up is an artificially synthesized promoter. We have validate this part through promoter strength assay using ''bgaB'' (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to our promoter strength assay in ''B. amyloliquefaciens'' NK-1 (showed in Figure 1), A2up is a very strong promoter in ''B. amyloliquefaciens'' NK-1.
 [[File:promoter3_NK.png]]