Difference between revisions of "Part:BBa K1804001:Design"
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+ | Shong, J., & Collins, C. H. (2013). Engineering the esaR promoter for tunable quorum sensing-dependent gene expression. ''ACS synthetic biology'', 2(10), 568-575. |
Revision as of 06:37, 18 September 2015
GFP under the control of constitutive promoter lacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 734
Design Notes
None
Source
The lacI promoter (plac) was obtained from the pAC-EsaR-EsaI plasmid (Shong & Collins, 2013), which was a gift from Cynthia Collins (Addgene plasmid # 47660).
The Green Fluorescence Protein (GFP) gene was obtained from BioBricks Part BBa_E0040 (https://parts.igem.org/Part:BBa_E0040).
References
Shong, J., & Collins, C. H. (2013). Engineering the esaR promoter for tunable quorum sensing-dependent gene expression. ACS synthetic biology, 2(10), 568-575.