Difference between revisions of "Part:BBa K1859003:Experience"
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When these devices were expressed in E.coli, Curcular mRNA including the linker was made.
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The efficiency that mRNA is circularized changed by sequence of the mRNA.
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859024" >[BBa_K1859024] </a>
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We let mRNA of RFP circularize and produced a long-chain protein, but the functionality was lost.
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is the generator to improve circular efficiency of mRNA including His-RFP.
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It is a problem to make a long-chain protein with keeping functionality.
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859029" >[BBa_K1859029] </a>
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is the generator that express long-chain protein with keeping functionality of the protein.
 
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Revision as of 07:56, 21 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1859003

User Reviews

UNIQ948fb63268184707-partinfo-00000000-QINU UNIQ948fb63268184707-partinfo-00000001-QINU

[iGEM gifu 2015] .

We attached this part[BBa_1859003] to 5 'sides of His-RFP without stop codon [BBa_K1332002] . Then, it was connected to each 5 'sides of [BBa_K1859004] and [BBa_K1859006] . In addition,Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] was conbined with them, making [BBa_K1859024] and [BBa_K1859029] . [BBa_K1859024] is the generator to improve circular efficiency of mRNA including His-RFP. [BBa_K1859029] is the generator that express long-chain protein with keeping functionality of the protein.